Résumé: Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to high-resolution live imaging has been mainly limited to fluorescent and stationary specimens. Here, we present advancements in SIM to jointly tackle all the challenges of imaging living samples, i.e., obtaining super-resolution over an undistorted wide-field while dealing with sample motion, multiple scattering, sample-induced optical aberrations, and low signal-to-noise ratio. By using adaptive optics to compensate for optical aberrations and a reconstruction algorithm tailored for moving and thick tissue, we successfully apply SIM to in vivo retinal imaging and demonstrate structured illumination ophthalmoscopy with optical sectioning and resolution improvement for in vivo imaging of the human retina.

Résumé: Understanding the rheological behavior of rapid granular flows is crucial for understanding various geological processes, such as fast fault slip and rapid motion of landslides. In this study, we conducted rotary shear experiments on different granular materials, spanning a range of shear velocities from slow to rapid and under varying normal stresses, to investigate the evolution of mechanical behavior under different flow conditions. The experimental results showed that steady-state shear resistance varied with normal stress and material composition at shear velocities below 1 m/s. A consistent velocity-dependent trend was observed. The steady-state shear resistance of the sample experienced a transition from velocity-strengthening behavior at low shear velocities (below 0.1 m/s) to velocity-weakening behavior at higher shear velocities (above 0.1 m/s). Interestingly, at shear velocities exceeding 1 m/s, the steady-state shear resistance became independent of normal stress and material composition, converging to a similar steady-state value for both crushable and uncrushable materials. Although normal stress and mineral composition had a limited influence on steady-state shear resistance at high shear rates, they significantly affected the weakening rate (the transition from peak strength to steady-state shear resistance), which was strongly correlated with the material's crushing ability, as characterized by the Weibull modulus. These findings provide insights into the mechanisms governing the hypermobility of mega-landslides and the rapid dynamics of geological flows.

Résumé: Tissue Doppler imaging (TDI) mode of echocardiography plays a crucial role in diagnosing several cardiac conditions by recording the myocardial contraction velocities. To mitigate the need of experts for performing echocardiography, several studies have proposed the use of Seismocardiogram as an easier alternative to measure the cardiac timings. However, exact correlation with tissue Doppler measured cardiac velocities has not been explored. Moreover, most of the applications only use single channel contact accelerometers on the chest, thus limiting their utility. In this work, we propose the use of a novel airborne ultrasound based surface motion camera (SMC) for non-contact multichannel recording of cardiac induced surface velocities from the chest. Validation study was conducted on 30 healthy subjects with simultaneous recordings of single channel ECG along with the chest surface velocities followed by clinical Echocardiography. The SMC recorded surface velocity waveforms show correlation similarity of over 0.6 with Tissue Doppler velocity waves extracted from the echocardiographic images. Discrete time warping based distance analysis was also performed to quantify their morphological similarity. Quantitative parameters including the peak systolic velocity and amplitude ratio extracted from the chest recordings show a linear correlation (R² greater than 0.8) with that of the TDI values. Additionally, multichannel recording allows visualization of the velocity profiles on the chest and efficiently captures the spatial variations for left ventricular and septal sites. Hence, this new modality shows the potential to be a vital diagnostic technique for non-contact and robust monitoring of the cardio-mechanical functions.